Detection and Differentiation of Potato Virus Y Strains by Melting Analysis of an Oligonucleotide Virus Probe
. American Journal of Potato Research 2016
, 620-625. Publisher's VersionAbstract
A novel simple, fast qualitative method for detection and differentiation of PVY strains in potatoes by the LightCycler technology is described. Fluorescent-labeled probe designed to contain variable degree of homology with a ‘target sequence’ of several known reference PVY strains was annealed to PCR products of these viruses followed by a graded melting analysis. The specific characteristics of the melting curves enable the detection, distinction and differentiation of each of following four known PVY strains O-FL, O-RB, N and NTN, in a single reaction obviating the need for size or nucleotide sequence analyses. In addition, it was demonstrated that virus extracts from plants infected with more than one strain can be resolved using this procedure. The melting-curves of extracts from 55 market size tubers harvested randomly from commercial fields and compared with those of the reference virus strains, revealed the presence of O-FL, O-RB and NTN strains of PVY. © 2016, The Potato Association of America.
Physiology of Domesticated Alliums: Onions, Garlic, Leek, and Minor Crops
; Encyclopedia of Applied Plant Sciences; 2016; Vol. 3, pp. 255-261. Publisher's VersionAbstract
More than 50 species of the genus Allium have been cultivated worldwide for sustenance, flavor, and medicinal purposes, as well as for ornamentals, from the tropics to the temperate zone. In most alliums, intact tissues contain odorless, nonvolatile sulfur-containing molecules that upon cells' disruption break down enzymatically to form volatile flavor compounds that are responsible for the characteristic odor and taste of these species. The genetic makeup, photoperiod, and both storage and growth temperatures are the main factors controlling bulbing and florogenesis in these plant species. The most common Allium food crops are bulb onion garlic, leek, shallot, chives, and Welsh onion. Most fertile cultivated alliums are raised from seed, transplants, or sets. However, some sterile species, such as garlic, elephant garlic, rocambole tree onion, topsetting onions, and shallot are propagated vegetatively. During millennia of cultivation, no natural co-evolution with biotic and abiotic agents has taken place. Hence, most cultivated alliums lack many important traits, including resistance to pests and environmental stresses. Current efforts and employment of both classical and novel tools for genetic and plant physiology studies are expected to accelerate improvements in terms of distribution, yield, and quality of these important crops. © 2017 Elsevier Ltd. All rights reserved.
; Encyclopedia of Applied Plant Sciences; 2016; Vol. 3, pp. 39-44. Publisher's VersionAbstract
A concise description of the history, classification, cytology, and botany of potato is presented. The effects of the environment on the morphology, physiology, and development of the potato plant and the adverse effects of high temperatures, drought, and salinity on growth, tuber development, and tuber quality are described. Tuber dormancy and its control in association with storage and with 'seed' tubers' vigor are discussed. Principles of crop production, storage, and tuber quality are presented. © 2017 Elsevier Ltd. All rights reserved.