JUST LOOK UP! Team Finds How Plants Make Aerial Roots
Roots are normally associated with things that live underground, in the damp and the dark. Think of turnips, radishes and yams. However, many plants make their roots above ground. Ivy uses its roots to climb on buildings and the mighty ficus tree uses them to support their large branches. What makes plants form roots in the “wrong place,” so to speak? That would be like us humans sprouting legs from our shoulders.
In a study published this week in the prestigious journal Science, Hebrew University of Jerusalem (HU) Professor Idan Efroni and his team found the hidden mechanism that enables aerial roots to happen. By decomposing the stem to individual cells, the team identified the extremely rare cells that, when conditions are ripe, cause roots to grow in the air.
“Superficially, these look like other plant cells which is why they evaded detection for so long,” Efroni explained. “We used new techniques to closely screen thousands of cells, one-by-one. We knew that by finding the cells that can make roots, we would be able to look for the ‘switch’ that turns them on.”
Plants make roots from small organs called meristems. By closely examining these unique cells, Dr. Naama Gil-Yarom, a research associate at the HU lab, was able to catch them in the act of making a meristem and to identify the genes that are active right at the transition point. One gene in particular stood out, and when the HU PhD student Moutasem Omary used CRISPR to delete this gene, the plants lost their ability to make aerial roots.
When Efroni and his team studied the genome, they were in for a surprise. Right next to the gene that controlled aerial roots production was a very similar gene. “We recognized it immediately from previous studies as the gene that controls the formation of underground roots,” shared Efroni, “I remember thinking that we have just stumbled upon the central hub that controls root formation.” Indeed, when the researchers disabled all of these genes, the plants could not grow any roots at all.
By tracing the evolution of these genes, the team found that many major crops, such as sweet potatoes, beans, tomato, rice, maize and wheat, share this dual root-control-system. “The ability to make aerial roots is highly advantageous to the plant,” explained Efroni. “In the event that the underground roots are flooded or damaged, the plant can grow aerial roots and survive the assault,” he added. Plants evolved this ability early on and never forgot how to do it.
Nevertheless, what is helpful in nature may actually come as a disadvantage in agriculture. Many plants are grafted, meaning they have the root system of one plant and the aboveground system of another. This allows farmers to grow plants that are resistant to soil disease. However, if the top part of the graft grows an aerial root, it will bypasses the soil-resistance and make all the effort of grafting useless. However, thanks to Efroni and his team’s discovery, we know which genes to target and can create plants with no aerial roots, making the practice of grafting that much more effective.
Looking ahead, the group plans to modify the DNA code at the root control cluster to make customized above- and below- ground root systems. As Efroni concluded, “here in Israel, to make the most use of the land we’ve got, we need to optimize the way our food crops grow and utilize resources. Doing that is a daunting and complex task, but, step by step, we’re getting there.”
CITATION: Moutasem Omary, Naama Gil-Yarom, Chen Yahav, Evyatar Steiner, Anat Hendelman, and Idan Efroni (2022). A conserved superlocus regulates above- and belowground root initiation. Science 375: eabf4368. 10.1126/science.abf4368
FUNDING: HHMI International Research Scholar; Israeli Science Foundation.
- Basic genetics and physiology studies of the spontaneous mutations of ripening inhibitor genes in tomatoes and the consequent exploitation of the knowledge resulted in the original development of long shelf-life tomatoes, thus revolutionizing the fresh market industry.
Abstracts of Publications (2005-2014)
Brown JK, Lambert GM, Ghanim M, Czosnek H and Galbraith DW (2005).
Nuclear DNA Content of the Whitefly Bemisia tabaci (Genn.) (Aleyrodidae: Homoptera/ Hemiptera) Estimated by Flow Cytometry. Bulletin of Entomological Research. 95:309-312.
Supplementary Data Table
Means of water loss rate (WLR), relative water loss (RWL), fruit fresh weight (FFW), pericarp surface area (PSA), pericarp weight per surface (PWS) and relative dry matter content (RDM) of 161 genotypes analyzed, grown for 2 years (2001-02 and 2002-03) in the Arava Valley of southern Israel.
Decoding the genetic basis of floret fertility in wheat
A high grain yield is undoubtedly a desirable trait in cereal crops. Floret fertility is a key factor
Abstracts of Current Research
Genomic/metabolomic/proteomic approaches for identification of novel (regulatory and biosynthetic) aroma genes
Site-specific genome modification and genetic engineering in plants
Visit the Peleg Lab Website
Wheat production under climate changes: The highly variable and unpredicted rainfall within and between seasons, which characterize the Mediterranean region, results in significant yield losses. Our research focuses on improving wheat production under changing climate.
Research in the Mosquna lab is focused on plants response to environmental stress; we are interested in the apex of abscisic acid (ABA) signal transduction pathway and related cellular/physiological stress response. In addition we are kin to discover novel plant hormones and growth regulators, by an unbiased approach monitoring plant small molecule-protein interactions. Study of this regulatory scaffold is expected to be of long-term benefit to biotechnology and lead to innovations in agriculture.
The role of Solanaceae aquaporins in improving plant vigor, abiotic stress tolerance and yield production.
The regulatory role of aquaporins in controlling plant cell water permeability.
Developing a high-throughput automated screening system for the on-line collection and analysis of data from many plants simultaneously. Pinpointing plants showing improved transpiration-use efficiency under normal and stress conditions at an early developmental stage.